Dr. Christian graduated from Appalachian State University in 2005 and received her Ph.D. in Biological Chemistry in 2010 from the University of North Carolina at Chapel Hill. Her graduate work focused on mechanisms of translation initiation in mammalian mitochondria. Brooke did her postdoctoral work at Yale University as an NIH postdoctoral fellow where she studied the contribution of mitochondrial reactive oxygen species to the neurodegenerative disease Ataxia-Telangiectasia. Dr. Christian joined the department of chemistry at Appalachian State University in 2015 and teaches Biochemistry I, Biochemistry II, Biochemistry I Laboratory, Introduction to Chemical Research, and General Chemistry I Laboratory.
Current research projects in the Christian lab are focused on cellular stress, specifically stress induced by mitochondrial reactive oxygen species (mtROS). The first project involves the contribution of mtROS in the assembly of oxidative phosphorylation complexes. Dr. Christian uses tissue culture and animal models to investigate mitochondrial superoxide dismutase and mitochondria-targeted catalase overexpression systems. The second project uses the same models to investigate adipocyte (fat cell) differentiation, with a long-term goal of targeting this process to help reduce childhood obesity. The third project tests the ability for tardigrade-specific proteins to stabilize protein-based therapeutics, with the hope of storing and shipping these drugs at room temperature. Techniques used in the Christian lab include tissue culture, western blotting, qPCR, PAGE (denaturing/SDS and blue native), shRNA knockdown of genes of interest, immunofluorescence, and subcellular fractionation.
Title: Assistant Professor
Email address: Email me
Phone: (828) 262-2961